Journal: Frontiers in Pharmacology

Article Title: Protectin DX alleviates lumbar radicular pain in rats by suppressing RIP3/MLKL-associated necroinflammation through restoration of autophagic function

doi: 10.3389/fphar.2026.1957244

Figure Lengend Snippet: Effects of GSK’872 on pain behaviours and spinal neuroinflammation in LRP rats. (A, B) GSK’872 significantly improved MWT and TWL in LRP rats (n = 9 rats/group). Data are presented as mean ± SEM. Two-way repeated-measures ANOVA was performed, followed by Tukey’s post hoc test (* p < 0.05 vs. sham group; # p < 0.05 vs. vehicle group). (C) Representative western blots showing the protein expression of RIP3 and MLKL (n = 4 rats/group). (F) Representative Western blots showing protein expression of IL-1β, IL-6, AND TNF-α (n = 3 rats/group). (J) Representative images of PI staining for necrotic cells (red) (n = 3 rats/group); scale bars: 100 µm. (D, E, G-I, K) Quantitative analyses of protein levels of RIP3 (D) , MLKL (E) , IL-1β (G) , IL-6 (H) , and TNF-α (I) , as well as PI-positive cell number (K) . Data are presented as mean ± SEM. Statistical comparisons were performed using one-way ANOVA followed by Tukey’s post hoc test (* p < 0.05 vs. sham group; # p < 0.05 vs. vehicle group). Abbreviations: IL-1β, interleukin-1β; IL-6, interleukin-6; LRP, lumbar radicular pain; MLKL, mixed lineage kinase domain-like protein; MWT, mechanical withdrawal threshold; PI, propidium iodide; RIP3, receptor-interacting protein kinase 3; DAPI, 4’,6-Diamidino-2-phenylindole; TNF-α, tumor necrosis factor-α; TWL, thermal withdrawal latency.

Article Snippet: Cryosections (10 μm) were prepared, washed with phosphate-buffered saline (PBS), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Boster, China) for 5 min at room temperature.

Techniques: Western Blot, Expressing, Staining

Journal: Frontiers in Pharmacology

Article Title: Protectin DX alleviates lumbar radicular pain in rats by suppressing RIP3/MLKL-associated necroinflammation through restoration of autophagic function

doi: 10.3389/fphar.2026.1957244

Figure Lengend Snippet: Effects of Protectin DX on pain behaviors and spinal necroinflammation in LRP rats. (A, B) PDX (10ng, 100ng or 200ng) treatment prominently meliorated the 50% MWT and prolonged TWL of LRP rat models (n = 9 rats/group). Data are presented as the mean ± SEM; Two-way ANOVA was used for comparisons among groups followed by Turkey’s multiple comparisons test (*P < 0.05 versus sham group; #P < 0.05 versus vehicle group; & P < 0.05 versus 10ng group). (C, F) Representative western blots showing protein expression of RIP3, MLKL, IL-1β, IL-6, and TNF-α (n = 4 rats/group). (J) Double immunofluorescence staining for MLKL (green) and PI (red) in the lumbar spinal cord on postoperative day 7 ((n = 3 rats/group). scale bars: 100µm. (D, E, G-I, K) Quantitative analyses of protein levels of RIP3 (D) , MLKL (E) , IL-1β (G) , IL-6 (H) , and TNF-α (I) , as well as MLKL/PI double-positive cell numbers (K) . Data are presented as mean ± SEM. Statistical comparisons were performed using one-way ANOVA followed by Tukey’s post hoc test (* p < 0.05 versus sham group; # p < 0.05 versus vehicle group; & p < 0.05 versus 10ng group). Abbreviations: IL-1β, interleukin-1β; IL-6, interleukin-6; LRP, lumbar radicular pain; MLKL, mixed lineage kinase domain-like protein; MWT, mechanical withdrawal threshold; PI, propidium iodide; RIP3, receptor-intracting protein kinase 3; DAPI, 4’ 6-Diamidino-2-phenylindole; TNF-α, tumor necrosis factor-α; TWL, thermal withdrawal latency; Protectin DX, PDX.

Article Snippet: Cryosections (10 μm) were prepared, washed with phosphate-buffered saline (PBS), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Boster, China) for 5 min at room temperature.

Techniques: Western Blot, Expressing, Double Immunofluorescence Staining

Journal: Reviews in Cardiovascular Medicine

Article Title: Targeting Tumor Necrosis Factor-α Mitigates Glucose Fluctuation-Induced Aortic Valve Fibrosis: Insights From Diabetic Rat Models

doi: 10.31083/RCM42804

Figure Lengend Snippet: Colocalization of CD3, CD68, and JAK1 in the aortic valves of diabetic rat models . (A,B) The coimmunostaining analysis of the HG and GF groups demonstrated the presence of CD3, CD68, and JAK1 proteins, indicating that JAK1 expression is primarily localized in T lymphocytes and macrophages. Notably, this expression pattern could be altered through the inhibition of TNF- α . Scale bar = 50 µm. DAPI, 4 ′ ,6-diamidino-2-phenylindole.

Article Snippet: In addition, the concentrated SABC-POD (Mouse/Rabbit IgG) kit (SA2010, BOSTER Biological Technology Co., Ltd., Wuhan, China), DyLight 488 conjugated AffiniPure goat anti-mouse IgG (H + L) (BA1126, BOSTER Biological Technology Co., Ltd., Wuhan, China), DyLight 594 conjugated AffiniPure goat anti-rabbit IgG (H + L) (BA1142, BOSTER Biological Technology Co., Ltd., Wuhan, China), ethylenediaminetetraacetic acid (EDTA) antigen retrieval solution (AR0023, BOSTER Biological Technology Co., Ltd., Wuhan, China), 4 ′ ,6-diamidino-2-phenylindole (DAPI) staining solution (AR1176, BOSTER Biological Technology Co., Ltd., Wuhan, China), human TNF- α enzyme-linked immunosorbent assay (ELISA) kit (EK0525, BOSTER Biological Technology Co., Ltd., Wuhan, China), and human TGF- β 1 ELISA kit (EK0513, BOSTER Biological Technology Co., Ltd., Wuhan, China) were obtained from Boster, China.

Techniques: Expressing, Inhibition